TNFAIP8在子痫前期胎盘组织中的甲基化情况及表达
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福建医科大学 省立临床医学院,福建省立医院 妇产科,福州350001

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收稿日期: 2019-07-16
基金项目: 福建省卫生计生科研人才培养项目(中青年骨干人才培养项目,2018-ZQN-22); 福建省立医院“创双高”火石基金项目(2020HSJJ19); 国家自然科学基金青年基金项目(81800363)作者简介: 林靓,女,主任医师,医学博士. Email: kitty342@163.com


DNA Methylation Profile and Expression of Tumor Necrosis Factor Alpha Induced Protein 8(TNFAIP8)in Preeclampsia Placenta
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Department of Gynecology and Obstetrics, Provincial Clinic College of Fujian Medical University,Fujian Provincial Hospital, Fuzhou 350001, China

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    摘要:

    目的探讨子痫前期胎盘组织中TNFAIP8甲基化情况及其在胎盘和外周血的表达水平。方法应用Illumina Human 450K甲基化芯片检测早发型组(EOPE组)、晚发型组(LOPE组)及对照组胎盘组织,焦磷酸测序验证TNFAIP8甲基化差别情况。采用RT-PCR检测TNFAIP8-mRNA,Western-blot检测TNFAIP8蛋白,进一步验证其在胎盘和外周血中的表达。结果与对照组比较,甲基化芯片检测和焦磷酸测序验证检测结果均提示LOPE组及EOPE组TNFAIP8甲基化水平均明显降低,差别有统计学意义(P<0.05); 而LOPE组与对照组比较,差别无统计学意义(P>0.05)。3组胎盘组织及外周血的TNFAIP8-mRNA及蛋白表达差别均有统计学意义(P<0.05)。结论TNFAIP8基因在EOPE胎盘组织中明显低甲基化。子痫前期胎盘及外周血中 TNFAIP8-mRNA及蛋白显著过表达,可能参与子痫前期发病机制。

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    ObjectiveTo explore the methylation profile and protein expression of TNFAIP8 in both the preeclampsia placenta and peripheral blood.MethodsThe placental tissues of early-onset preeclampsia(EOPE), late-onset preeclampsia(LOPE)and normal pregnant group(NP)were tested by Illumina Human 450K methylation beadchip.Differences in TNFAIP8 methylation profile among three groups were verified by pyrosequencing.The expression levels of TNFAIP8 mRNA and protein expression were examined by RT-PCR and Western-blot analysis.ResultsBoth the methylation beadchip analysis and pyrosequencing showed that TNFAIP8 hypomethylation in EOPE group was significantly different compared with LOPE group or NP group(P<0.05).However, there was no significant difference between LOPE group and NP group(P>0.05).The three groups have statistically significant difference in TNFAIP8 mRNA and protein expression(P<0.05).ConclusionsSignificant hypomethylation of TNFAIP8 occurs in placenta of early-onset preeclampsia.NFAIP8 mRNA and protein overexpression are probably associated with preeclampsia pathogenesis.

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林靓, 柳之彦, 林容, 阮舆鑫, 董洁琼, 郑晶. TNFAIP8在子痫前期胎盘组织中的甲基化情况及表达[J].福建医科大学学报自然版,2020,(4):255-259

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