Objective Establishing a platform to evaluate the value of fluorescence in stiu hybridization for detecting Y-chromosome microdeletion. Methods One or two biopsies from six to eight cells at the cleavage stage were used to perform the fluorescence in stiu hybridization. The embryo whose karyotype was 46, XX was transferred. Results Eleven embryos biopsies were observed, among which 8 were 46, XX, 2 were abnormal and only one was 46, XY. After two embryos of karyotype 46, XX were transferred, one healthy baby girl was born. Conclusions Fluorescence in stiu hybridization is effective in preventing the next generation from inheriting genetic defects and has crucial role in preimplantation genetic diagnosis.