ObjectiveTo evaluate the value of two-dimensional combined with three-dimensional ultrasound (2D＆3D-US) on diagnosing the dysplasia of the fetal cerebral sulcus and gyrus.Methods2D-US was performed to record the growth of cerebral sulcus and gyrus of 66 fetuses, who were suspicious central nervous system dysplastic.Then 3D-US were performed for volume scan in three orthogonal planes mode display and storage, offline diagnostics, and comparing the diagnosis and follow-up results.ResultsCompared to the follow-up or autopsy, 36 fetuses proved to be 2cerebral sulcus and gyrus dysplasia or combine with it.Of them, 10 fetuses were agyric, 26 fetuses were hypoplasia, and the rest 30 fetuses were normal.The diagnose accordance rate of 2D＆3D and 2D-US were 92.42%（61/66）and 75.76%(50/66), respectively.There was statistically significant difference between them （χ2=6.68，P＜0.01）.Conclusions2D＆3D-US could promote the accurate diagnosis in dysplasia of fetal cerebral sulcus and gyrus, which has an important clinical value.