ObjectiveTo establish mouse models of experimental autoimmune encephalomyelitis (EAE) induced by peptide myelin oligodendrocyte glycoprotein (MOG3555) and study their pathological characterization.MethodsThe female EAE model of C57BL/6 mice (10~12 weeks) were immunized subcutaneously at four sites into the flanks with 300 μg of myelin oligodendrocyte glycoprotein peptide (MOG3555) with the assistance of Complete Freund’s Adjuvant(CFA) and Pertussis toxin (PTX).We observed the clinical symptoms, histopathologic changes and changes on magnetic resonance scan.ResultsThe experimental group developed the typical symptoms of EAE on （12±4） days after immunization with the incidence of 83.3% and showed a chronic monophasic course.There was a large number of inflammatory cells infiltration and demylination inthe lumbar spinal cord.Electron micrographs demonstrated a considerable amount of the myelin sheaths displayed loose, vacuoles and splitting.Intramedullary spinal MRI study showed patchy T2 hyperintensityin EAE mice.ConclusionOur study reports a MOGinduced EAE model that has a high incidence and its pathologic changes were similar to multiple sclerosis (MS), so it might be an ideal model for the research on MS.