Objective To investigate the expression of Cyclin A in gastric cancer and to assess the correlation of Cyclin A to prognosis of patients gastric cancer. Methods 125 cases of gastric cancer patients at Fujian Medical University Union Hospital [JP+3]were analyzed retrospectively. We examined the expression of Cyclin A by immunohistochemistry. The survival rate was calculated by the Kaplan-Meier method, the Log-rank test and Cox’s proportional hazards regression model using SPSS 16.0. Results There were 78 patients (62.4%) with the positive expression level of Cyclin A in gastric cancer. Compared with the negative group, cases in the positive group have poor pathologic types, larger tumor, and more advanced cancer. The 5-year survival rate in patients with positive and negative Cyclin A expression was 30.8% and 72.3%, respectively, with the difference being statistically significant (P<0.05). Univariate survival analysis showed that the tumor size, Lauren types, pathologic types, depth of tumor invasion, lymph node metastasis, TNM stage, and Cyclin A were associated with the prognosis. Multivariate analysis demonstrated that depth of tumor invasion, lymph node metastasis, and TNM stage were independent risk factors for the prognosis (P<0.05). Conclusion Patients with positive expression of Cyclin A are prone to poor pathologic changes andadvanced cancer, leading to worse outcomes. Cyclin A may be identified as a biomarker for predicting the prognosis of gastric cancer.