Objective To establish a quality control method by using reversed-phase high performance liquid chromatography coupled with DAD detector(RP-HPLC-DAD)to simultaneously determine five components(puerarin, liquirtin, isoliquiritin, daidzein, glycyrrhizic acid)in Yuquan pills. Method Chromatographic experiment was carried out on an Ultimate^TM XB-C18 column(250×4.6 mm, 5 μm)with a gradient elution composed of acetonitrile(B)and 0.2% formic acid(A). The flow rate was 1.0 mL/min and the detection wavelength were 254, 280, 340 nm. The column temperature was set at 30 ℃. Results The calibration curves of five components(puerarin, liquirtin, isoliquiritin, daidzein, glycyrrhizic acid)showed good linearity in their concentration ranges with r≥0.997. The average recovery was 87.5%～104.3% with relative standard deviation(RSD)less than 3.3%(n=6). Conclusion The method is rapid, simple, and with good repeatability and stability, and it can be used in quality control of Yuquan pills.