Objective By exploring the correlation between carotid intima-media thickness(CIMT), ankle-brachial index(ABI), biochemical indicators and the severity of patients with femoral popliteal arteriosclerosis obliterans(ASO),to provide a certain basis for the etiological research of femoral popliteal arteriosclerosis obliterans and its prevention and treatment. Methods 151 patients with femoral popliteal arteriosclerosis obliterans were divided into three groups based on the severity. The general data, CIMT values, ABI values, total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL), high density lipoprotein(HDL), fasting blood glucose(FBG), total bilirubin(TBIL), uric acid(UA), plasma fibrinogen original(FIB), and glomerular filtration rate(GFR)were collected and retrospectively analyzed by Chi Square Test, One-Way ANOVA or Spearman Rank Correlation. Results The differences in age, prevalence rate of hypertension, prevalence rate of diabetes, CIMT, ABI values, TG, UA, FIB and GFR were statistically significant(P=0.004,0.003,0.046,<0.001,<0.001,0.026,0.034,0.029,0.001)among the severity groups. There were no significant differences in gender, prevalence rate of coronary heart disease, prevalence rate of cerebral infarction, the proportion of smoker, TC, LDL, HDL, FBG, and TBIL among the three groups. The severity of femoral popliteal ASO was positively related to CIMT value, age and renal failure(r_s=0.371, 0.244, 0.254), while negatively related to ABI value(r_s=-0.528). Conclusions The severity of femoral popliteal ASO was related to various risk factors and indexes. Therefore, combination with these indexes can possibly discover ASO patients as early as in asymptomatic stage. Timely intervention can reduce the rate of morbidity and disability of ASO.