Objective To explore the effect of microRNA-383 on angiogenesis and its molecular mechanism. Methods Bioinformatic prediction was performed to find the target genes of miR-383. Then the target genes associated with angiogenesis were selected as the candidate genes for luciferase assays. Fluorescence quantitative polymerase chain reaction(qPCR)were performed to measure the mRNA level of endogenous VEGFA expression in HUVEC cells affected by overexpression of miR-383. Protein level of endogenous VEGFA was tested by ELISA and Western-blot assays. In vitro angiogenesis assay was performed to verify the effects of miR-383 mediated downregulation of VEGFA on angiogenesis. Results Bioinformatics prediction showed that miR-383 targeted multiple genes related to angiogenesis, such as VEGFA, FGF9. The luciferase assay confirmed that miR-383 could inhibit VEGFA expression by binding to its 3'-UTR. qPCR, while ELISA and Western-blot assay revealed that overexpression of miR-383 reduced VEGFA expression only at protein levels. In vitro matrigel based angiogenesis assay showed that overexpression of miR-383 reduced angiogenesis by downregulation of VEGFA. Conclusion miR-383 can downregulates VEGFA expression and reduces angiogenesis.