Objective To evaluate the association of catalase(CAT)single nucleotide polymorphism with the dental fluorosis. Methods 195 patients and control subjects were recruited from Zhijin County, Guizhou province with endemic coal-burning fluorosis. Information of participants was collected by questionnaire and genotype of CAT rs769217 and rs2300182 was detected by TaqMan method. Results Although the univariate and multivariate logistic regression analysis showed that genetic model of rs769217 and rs2300182 were not associated with dental fluorosis, Stratified and interaction analyses showed that the risk of dental fluorosis for the AT+TT genotypes combined versus the AA genotype was evidently decreased in individuals with higher calcium intake(≥482 mg/d), but not in individuals with low calcium intake(<482 mg/d). There were an interactions between rs769217 polymorphism and the dietary intake of calcium related to dental fluorosis(P<0.05). Conclusion The CAT rs769217 polymorphism may be associated with a decreased risk of dental fluorosis in coal-burning fluorosis area of Guizhou, dependent on dietary calcium intake. But there is not an association between CAT rs2300182 polymorphism and dental fluorosis.