Objective To establish a HPLC method for the simultaneous determination of the four effective components(neopperidin, naringin, hesperidolide hydrate, and poncirin)in Daidai flavonoids lipid-lowering extract. Methods We used high performance liquid chromatography and chromatographic column Lichrocart C18(250 mm×4.6 mm, 5 μm). Acetonitrile-----------------------------------------------0.2% acetic acid solution was used as the mobile phase. Gradient elution was used, with flow rate 1.0 mL/min, detection wavelength 284 nm, column temperature 30 ℃, and injection volume 10 μL. External standard method was established for the simultaneous determination of the contents of neohesperidin, naringin, Hesperidin Hydrate, and poncirin in Daidai flavonoids lipid-lowering extract. Results The concentration of nehesperidin, naringin, hesperidate lactone hydrate, and poncirin were linear in the range of 76.365-1 491.482 μg/mL(r=0.999 4), 49.155-1 024.283 μg/mL(r=0.999 7), 5.134-61.151 μg/mL(r=0.999 5), 1.771-40.962 μg/mL(r=0.999 8)respectively. The average recovery rates(n=6)were 100.3%, 102.2%, 97.98%, and 95.16%, respectively. The relative standard were 1.50%, 1.79%, 1.70%, 2.42%, respectively. Conclusion The established HPLC method for simultaneous determination of the four effective components in the extracts can be used to quantitatively monitor the quality of Daidai flavonoids lipid-lowering extract.