Objective Compare the difference of low density lipoprotein cholesterol(LDL-C)in stroke patients between a point-of-care device CardioChek PA analyzer and ADVIA2400 automatic analyzer, and to evaluate the correlation and deviation between them. Methods Blood samples were collected in the morning from 82 patients of noncardioembolic ischemic stroke in the department of neurology during April to June 2015. The total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C), and LDL-C were detected by CardioChek PA analyzer from the periphery or elbow venous blood samples immediately, and then detected by Siemens ADVIA2400 automatic analyzer from venous serum. The correlation coefficient and linear regression equations as well as rates of hyperlipidemia and bias were calculated,and sigma performance verification were used to evaluate the testing projects. Results The outcome showed that the values of LDL-C were(2.29±0.99)mmol/L(peripheral blood)and(2.20±1.00)mmol/L(venous blood)as detected by CardioChek PA analyzer, and these values were lower than that of ADVIA2400 automatic analyzer which is(2.92±1.02)mmol/L(P<0.05). The values of LDL-C did not have significant difference between peripheral blood and venous samples by CardioChek PA analyzer. Linear regression analysis showed the correlation coefficients of LDL-C were 0.861 and 0.831 between peripheral blood or venous samples by CardioChek PA analyzer and ADVIA2400 automatic analyzer(P<0.001). The rates of high TC, high TG and low HDL-C by CardioChek PA analyzer were similar to those by ADVIA2400 automatic analyzer(P>0.05), but the rates of high LDL-C were lower than those by ADVIA2400 automatic analyzer(P<0.05). Sigma performance verification showed that test of LDL-C by CardioChek PA analyzer was unacceptable(σ<2). Conclusion Different blood collection methods had no effect on LDL-C results by CardioChek PA analyzer. The LDL-C values of CardioChek PA were lower than those of ADVIA2400 automatic analyzer, which indicated that prevention and treatment of ischemic stroke still needs to refer to the LDL-C values measured by automatic biochemical analyzer.