AABSTRACT: Objective A mouse testicular injection model was established to obtain a simple, efficient and high survival rate method of protein inhibition, which can be used as a reference for related experiments and studies of spermatogenesis in mice. Methods The testes of mice were exposed by abdominal operation. Protein inhibitors were injected into mouse testes. The wound was sutured intermittently. Results Histological results showed that the chromosomes of spermatocytes in seminiferous tubules of the inhibition group were misaligned. The spermatocytes were arrested at metaphase and could not divide normally. This model can inhibit the proteins in the testes of mice, and the survival rates of mice were high. Discussions The commonly used method of testicular inhibition in mice is direct injection through the scrotum, however, the time window for operation is limited. Testicular injection through abdominal surgery in mice can achieve testicular inhibition in the 3-week-old mice with high success rate and good effects on protein inhibition. This method is suitable for the studies of male meiosis in spermatocytes. KEY WORDS: abdominal surgery; mouse testis; inhibition model; spermatogenesis